Mutational analysis of p53 gene in cervical cancer and useful polymorphic variants in exons 3 and 4

Abstract Background Factors contributing to the pathogenesis and progression of cervical cancer include poor attitude screening health intervention, late presentation, among others. Mutations in p53 gene have been attributed several cases. The present study was designed find relationships between mutation patterns carcinoma staging. Such knowledge could contribute early diagnosis cancer. Results From sequence analysis fragment isolated by polymerase chain reactions (PCR), nineteen (19) polymorphic variants were identified. Missense mutations occurred 47% samples, 32% silent mutations, 16% frameshift 5% nonsense mutations. Socio-biological characteristics participants revealed that 60% husbands with multiple sexual partners only 23.3% ever had Papanicolaou (Pap) smear test prior diagnosis, whilst 20% unaware test. Conclusions Increased severity staging as from histopathological found be associated accumulation higher levels gene. Molecular may prove useful a biomarker for